Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
نویسندگان
چکیده
منابع مشابه
Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
BACKGROUND The hybridization of synthetic Streptococcus pneumoniae tmRNA on a detection microarray is slow at 34 degrees C resulting in low signal intensities. RESULTS We demonstrate that adding specific DNA helper oligonucleotides (chaperones) to the hybridization buffer increases the signal strength at a given temperature and thus makes the specific detection of Streptococcus pneumoniae tmR...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2010
ISSN: 1472-6750
DOI: 10.1186/1472-6750-10-34